REAL TIME PCR DETECTION TEST KIT Porcine epidemic diarrhea virus deltacoronavirus and Transmissible gastroenteritis virus

qPCR kit that allow detecting and differenciatign Porcine epidemic diarrhea virus, Porcine deltacoronavirus and Transmissible gastroenteritis virus

DESCRIPTION:
This kit is a qPCR assay that allows detecting Porcine epidemic diarrhea virus, Porcine deltacoronavirus and Transmissible gastroenteritis virus genome in porcine sample.

SUMMARY AND EXPLANATION:
Porcine viral diarrhea is a major concern for the world pig farming industry and can result in substantial losses. Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is characterized by severe enteritis, watery diarrhea, vomiting, and dehydration. Although pigs of all ages are at risk of acquiring the infection, PED has a highest mortality rate among suckling piglets. Another virus called transmissible gastroenteritis virus (TGEV) also causes severe gastroenteritis similar to PEDV and belongs to the same virus family with PEDV. Porcine deltacoronavirus (PDCoV), a novel genus of Coronaviridae was first reported in Hong Kong in 2009 and 2010. As PEDV and TGEV, PDCoV is enteropathogenic in pigs because diarrhea and vomiting was observed in experimentally PDCoV-infected piglets. Diagnostic tools are needed to detect theses pathogens from clinical samples since all three coronaviruses cause similar clinical symptoms early in the disease, making it impossible to identify without testing which specific virus is at the root of the infection on the farm. Real-time quantitative RT-PCR is an accurate and sensitive method, allowing high-throughput detection and quantitation of viral loads using small volumes.

CHARACTERISTICS:
- Test per kit: 50 samples
- qPCR Results within two hours