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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | Target: 100 reactions - FAM Target + IC: 100 reactions - FAM, HEX Monodose Target: 24 qPCR tubes - FAM Monodose Target + IC: 24 qPCR tubes - FAM, HEX |
Description
Background:Mycobacterium leprae detection by using qPCR.
Mycobacterium leprae is a Gram-positive, intracellular, microaerophilic, acid-fast, pathogenic bacterium that causes leprosy. With a doubling time of 14 days, M. leprae has the longest doubling time of any studied bacteria. The incubation period of M. leprae can range between nine months and twenty years. The infection is thought to be spread through the skin and nasal mucosa. It replicates intracellularly inside histiocytes and nerve cells and has two forms. One form is tuberculoid, which induces a cell-mediated response that limits its growth. The second form of leprosy is the lepromatous form, marked by no-host resistance and thus direct killing of self-cells through the immune response. Common results of the immune response are loss of sensation and disfiguration. Chronic inflammation can lead to this paralysis, specifically along the periphery, and can affect facial regions, contributing to factors such as blindness.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Mycobacterium leprae is a Gram-positive, intracellular, microaerophilic, acid-fast, pathogenic bacterium that causes leprosy. With a doubling time of 14 days, M. leprae has the longest doubling time of any studied bacteria. The incubation period of M. leprae can range between nine months and twenty years. The infection is thought to be spread through the skin and nasal mucosa. It replicates intracellularly inside histiocytes and nerve cells and has two forms. One form is tuberculoid, which induces a cell-mediated response that limits its growth. The second form of leprosy is the lepromatous form, marked by no-host resistance and thus direct killing of self-cells through the immune response. Common results of the immune response are loss of sensation and disfiguration. Chronic inflammation can lead to this paralysis, specifically along the periphery, and can affect facial regions, contributing to factors such as blindness.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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