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Real Time PCR Detection Kit Escherichia coli virulence factor genes Panel (Fimbria F5 (K99) Fimbria F17 and Fimbria F41)

Brand: GPS
Virulence fimbrial genes from Escherichia coli F5, F17 and F41 detection test kit by using qPCR. Most E. coli strains are harmless, but some serotypes are pathogenic and can cause serious food poisoning in animals and humans. F5, F17 and F41 are relevant fimbrial genes related to enterotoxigenic and uropathogenic strains of E. coli.
SKU: GPCRsEscColPanel3
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Products specifications
TechnologyqPCR / Real Time PCR/ PCR
Sizes3 targets: 3 x 100 reactions - FAM
3 targets + IC: 3 x 100 reactions - FAM, HEX
Monodose 3 targets: 3 x 96 qPCR tubes - FAM
Monodose 3 targets + IC: 3 x 96 qPCR tubes - FAM, HEX
Background:Virulence fimbrial genes from Escherichia coli F5, F17 and F41 detection by using qPCR.
Escherichia coli is a Gram-negative, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms. Most E. coli strains are harmless, but some serotypes are pathogenic and can cause serious food poisoning in animals and humans. Fimbriae are adhesion organelles expressed by many Gram-negative bacteria. They facilitate adherence to mucosal surfaces and inflammatory cells and are considered as virulence factors of E. coli. F5, F17 and F41 are relevant fimbrial genes related to enterotoxigenic and uropathogenic strains of E. coli.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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Detection test kit of 10 different virulence genes from Escherichia coli by using qPCR. Some E. coli are pathogenic, meaning they can cause illness, either diarrhea or illness outside of the intestinal tract. The types of E. coli that can cause diarrhea can be transmitted through contaminated water or food, or through contact with animals or persons. Virulence genes detected include the Aerobactin synthase (iucC), Ligand-gated channel protein (fyuA), Ferric enterobactin receptor (iroN) and Aerobaction siderophore ferric receptor (iutA), all of them involved in iron transport under iron-stressed conditions; Temperature-sensitive hemagglutinin tsh autotransporter (tsh) can act both as an adhesin and as a serine protease; Colicin-V immunity protein (cvi) protects a cell against colicin V; Protein iss (iss) increases serum survival and confers group II surface exclusion; Chaperone protein (fimC) required for the biogenesis of type 1 fimbriae; ompT protein (ompT) aspartyl protease found on the outer membrane and Hemolysin F (hlyF) fatty-acyl-CoA reductase activity.

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