Real Time PCR Detection Kit Escherichia coli O157:H7

Background:Detection of Escherichia coli O157:H7 by using qPCR.
Escherichia coli O157:H7 is an enterohemorrhagic strain of the bacterium Escherichia coli and a cause of illness through food. Infection may lead to hemorrhagic diarrhea, and to kidney failure. Transmission is via the fecal-oral route, and most illness has been through undercooked, contaminated ground beef or ground pork being eaten. The “O” in the name refers to the cell wall (somatic) antigen number, whereas the “H” refers to the flagella antigen. Other serotypes may cause (usually less severe) illness. E. coli O157:H7 was first recognized as a pathogen as a result of an outbreak of unusual gastrointestinal illness in 1982.E. coli O157:H7 is markedly different from other pathogenic E. coli, as well. In particular, the O157:H7 serotype is negative for invasiveness (Sereny test), adheres through the E. coli common pilus, and does not produce heat-stable or heat-labile toxins.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
-Standard Template, dehydrated target copies for positive control