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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | Target: 100 reactions - FAM Target + IC: 100 reactions - FAM, HEX Monodose Target: 24 qPCR tubes - FAM Monodose Target + IC: 24 qPCR tubes - FAM, HEX |
Description
Background:Clostridium difficile detection by using qPCR.
Clostridium difficile is a Gram-positive, anaerobic, motile, ubiquitous, and spore-forming bacteria from the family Clostridiaceae. Sometimes antibiotic therapy for various infections has the adverse effect of disrupting the normal balance of the gut flora, in which case C. difficile may opportunistically dominate, causing Clostridium difficile colitis. Pathogenic C. difficile strains produce multiple toxins. The most characterized are enterotoxin (Clostridium difficile toxin A) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation in infected patients. Most cases of Clostridium difficile colitis are nosocomial infections.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Clostridium difficile is a Gram-positive, anaerobic, motile, ubiquitous, and spore-forming bacteria from the family Clostridiaceae. Sometimes antibiotic therapy for various infections has the adverse effect of disrupting the normal balance of the gut flora, in which case C. difficile may opportunistically dominate, causing Clostridium difficile colitis. Pathogenic C. difficile strains produce multiple toxins. The most characterized are enterotoxin (Clostridium difficile toxin A) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation in infected patients. Most cases of Clostridium difficile colitis are nosocomial infections.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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