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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | Target: 100 reactions - FAM Target + IC: 100 reactions - FAM, HEX Monodose Target: 24 qPCR tubes - FAM Monodose Target + IC: 24 qPCR tubes - FAM, HEX |
Description
Background:Caprine arthritis encephalitis provirus detection by using qPCR.
Caprine arthritis encephalitis virus is an enveloped, single-stranded RNA lentivirus belonging to the family Retroviridae. As part of their life cycle, once the viral RNA enters the cytoplasm DNA is produced by using its own reverse transcriptase. The new DNA is then incorporated into the host cell genome by an integrase enzyme, at this point, the retroviral DNA is referred to as a provirus. The virus adversely affects the immune system of its host, causing a disease known as caprine arthritis encephalitis, which is widely disseminated in goat herds on different continents. Common symptoms include arthritis, pneumonia, indurative mastitis, and encephalitis. However, many goats will not show any symptoms. By far, the most important source of infection is the milk of positive animals. In fact, if the kids are fed with communal milk from the herd, all the kids can be infected by just a single positive goat. As an effective vaccination does not exist, eradication programs are essential as well as fast detection of infected animals.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Caprine arthritis encephalitis virus is an enveloped, single-stranded RNA lentivirus belonging to the family Retroviridae. As part of their life cycle, once the viral RNA enters the cytoplasm DNA is produced by using its own reverse transcriptase. The new DNA is then incorporated into the host cell genome by an integrase enzyme, at this point, the retroviral DNA is referred to as a provirus. The virus adversely affects the immune system of its host, causing a disease known as caprine arthritis encephalitis, which is widely disseminated in goat herds on different continents. Common symptoms include arthritis, pneumonia, indurative mastitis, and encephalitis. However, many goats will not show any symptoms. By far, the most important source of infection is the milk of positive animals. In fact, if the kids are fed with communal milk from the herd, all the kids can be infected by just a single positive goat. As an effective vaccination does not exist, eradication programs are essential as well as fast detection of infected animals.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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