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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | Target: 100 reactions - FAM Target + IC: 100 reactions - FAM, HEX Monodose Target: 24 qPCR tubes - FAM Monodose Target + IC: 24 qPCR tubes - FAM, HEX |
Description
Background:Avian influenza type A virus detection by using qPCR.
Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses belonging to the Orthomyxoviridae family. Influenza type A virus is responsible for severe respiratory disease with high morbidity and low mortality. The several subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase).
There are seventeen different H antigens (H1 to H17) and nine different N antigens (N1 to N9). The H9N2 strain is a subtype of the Influenza A virus with a wide host range, adapted to both poultry and mammals, and with an extensive gene reassortment. In China, which is regarded as an epicenter of avian influenza viruses, the H9N2 virus has been detected in multiple avian species and several children. The antigen N2 is also associated with the subtypes H3N2 and H7N2, which can cause pneumonia in humans. The assay is focused on the detection of strains from the Arabian Peninsula and N2 associated mainly with the H9 subtype.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
Influenza A viruses are negative-sense, single-stranded, segmented RNA viruses belonging to the Orthomyxoviridae family. Influenza type A virus is responsible for severe respiratory disease with high morbidity and low mortality. The several subtypes are labeled according to an H number (for the type of hemagglutinin) and an N number (for the type of neuraminidase).
There are seventeen different H antigens (H1 to H17) and nine different N antigens (N1 to N9). The H9N2 strain is a subtype of the Influenza A virus with a wide host range, adapted to both poultry and mammals, and with an extensive gene reassortment. In China, which is regarded as an epicenter of avian influenza viruses, the H9N2 virus has been detected in multiple avian species and several children. The antigen N2 is also associated with the subtypes H3N2 and H7N2, which can cause pneumonia in humans. The assay is focused on the detection of strains from the Arabian Peninsula and N2 associated mainly with the H9 subtype.
Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control
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