Real Time PCR Detection Kit Actinobacillus pleuropneumoniae omlA-genotype Panel

Background:The panel comprises a series of species-specific targeted reagents designed for detection of the five variants of the membrane protein omlA of the Actinobacillus pleuropneumoniae (omlA I, omlA II, omlA III+IV, omlA IV and omlA V) by using qPCR.
Actinobacillus pleuropneumoniae (previously Haemophilus pleuropneumoniae), is a non-motile, Gram-negative, encapsulated coccobacillus bacterium found in the Pasteurellaceae family. It is a respiratory pathogen found in pigs and was found to be the causative agent for up to 20% of all bacterial pneumonia cases in swine. The main disease associated with this bacterium is porcine pleuropneumonia, a highly contagious respiratory disease, affecting primarily young pigs (usually less than 6 months). The symptoms include respiratory distress, bloodstained discharge (usually frothy) from the mouth, fever, anorexia, mild diarrhea, cyanosis, lethargy, and spontaneous abortion in sows. Mortality rates can reach 20-80 % in fattening pigs, with similarly high morbidity. Pigs that do survive the disease remain as carriers and spread the bacterium to other swine. A. pleuropneumoniae has a profound economic impact on pork production and pig farmers. Two biotypes and at least 15 serotypes are present and all of the can cause disease. The virulence potential between serotypes is due to the different expressions of Apx toxins and other virulence factors. The gene omlA is a virulence factor coding for an outer membrane protein. Due to omlA nucleotide sequence differences, isolates can be separated into five groups related with the different serotypes. As the sequences of the omlA III and omlA IV are highly conserved, omlA III positives must be inferred from two different tests. Positives for omlA III are positive for omlA III+IV and negatives for omlA IV.

Product Features:
-Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
-Resuspension buffer
-DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control