Menu
Search
Personal menu
EN
PT
EN
EN
PT
Search store
Close
Search
My account
My account
Close
Register
Log in
Wishlist
(0)
Products
Menu
Close
Products
Back
Equipments
Back
Agitators - Incubators - Vortexes
Autoclave Dry Heat Sterilizer
Automatic Pipettors and Liquid Dispensers
Baths and Heating Thermoblocks
Centrifuges
Electrodes (pH - conductivity - oxygen)
Electrophoresis
ELISA Tests
Freezers and Refrigerators
Homogenizers
Laboratory Balances
Lateral Flow Tests
Microbiological Incubators and Chambers
Micropipettes and Electronic Pipettes
Microscopes
PCR and Laminar Flow Chambers
Real Time PCR Tests
Spectrophotometers
Ultrasounds and Sonicators
Water Purifiers
Food
Back
Beverages Quality
Food Adulteration
Food Allergens
Food Safety
Genetically Modified Organisms (GMO)
Mycotoxins
Plant Pathogens
Water Quality
Other Food Tests
Human
Back
Bacteria in Humans
Covid-19 Tests
Fungi in Humans
Parasites in Humans
Virus in Humans
Other Tests for Humans
General Laboratory Solutions
Back
Antibiotics - Enzymes - Buffers
Cloning and Cell Research
Culture Media
DNA/RNA Amplification and Analysis
DNA/RNA Extraction and Purification
Plasticware
Veterinary
Back
Bacteria
Fungi
Hormones
Parasites
Virus
Other Tests for Animals
About Us
Services
Blog
Share:
Share on Twitter
Share on Facebook
Share on Pinterest
Home
/
Food
/
Food Allergens
/
Gliadin/Gluten ELISA
Gliadin/Gluten ELISA
Brand:
Diagnostics
Enzyme immunoassay for the quantitative determination of Gliadin/Gluten in food
SKU:
DEGLUE02
Register or Log In in order to request a quote
Register
Login
Products specifications
Technology
ELISA
Sizes
96 tests
Enzyme immunoassay for the quantitative determination of Gliadin/Gluten in food.
Background:
Gluten is the main part of the protein fraction of cereals and consists of nearly the equal amount of the protein compounds prolamin (gliadin) and glutenin. Because of its special physico-chemical attributes and its low price, gluten is not only contained in cereal products, but also in other food as sausage products and ice cream or in drugs and cosmetics as binder and filler.
For some persons, gluten has a pathological effect (coeliac disease). These people need to have a strict gluten free diet. In the European Union a maximum level of 20 ppm gluten is allowed for products declared as “gluten-free”, and 100 ppm gluten for products declared as “very low gluten” respectively. Sensitive detection systems are required to determine gluten residues in foodstuff.
Gliadin/Gluten ELISA represents a highly sensitive detection system and is particularly capable of the quantification of gliadin/gluten residues in bakery products, baby food, beer meat and chocolate.
Description:
The Gliadin/Gluten quantitative test is based on the principle of the enzyme-linked immunosorbent assay. An antibody directed against gliadin – the soluble fraction of gluten - is bound on the surface of a microtiter plate. Gliadin containing samples or standards are given into the wells of the microtiter plate. After 20 minutes of incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugated second antibody directed against gliadin is given into the wells and after 20 minutes of incubation, the plate is washed again. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of gliadin is directly proportional to the colour intensity of the test sample. Because of the equal amounts of gliadin and glutenin in wheat gluten, the gluten concentration of the sample is calculated by multiplication with the factor 2.
Product Features:
- The kit contains reagents for 96 determinations;
- Microtiter plate consisting of 12 strips with 8 breakable wells;
- Gliadin Standards: 0, 2, 6, 20, 60 ppm;
- ELISA reader at 450 nm;
- Standard range: 2 - 60 ppm;
- Sensitivity analytical: 0.3 ppm.
Similar Products
Beta-Lactoglobulin ELISA
Enzyme immunoassay for the quantitative determination of beta-Lactoglobulin in food
1.0000000097019166
Ovalbumin ELISA
Enzyme immunoassay for the quantitative determination of Ovalbumin in food
1.0000000097019166
Lysozyme ELISA
Enzyme immunoassay for the quantitative determination of Lysozyme in food
0.9096372425556183
Casein ELISA
Enzyme immunoassay for the quantitative determination of bovine Casein in food
0.886733777821064
Hazelnut ELISA
Enzyme immunoassay for the quantitative determination of Hazelnut in food
0.8837519660592079