Free Estriol ELISA

>Background:Since the production of estriol in pregnant women depends on a healthy maternal-placental-fetal system, the estriol concentration is a marker of both placental and fetal normal development and metabolism; hence the determination of serum or saliva estriol concentration is instrumental for the assessment of fetus health in advanced pregnancy . In non-pregnant women and men, estriol levels are low. Notwithstanding, one common application of salivary tests is the monitor- ing of the estriol levels in women undergoing hormone replacement therapy. Due to the significant temporal fluctuations in the concentrations of this hormone, multiple tests are recommended to obtain reliable results.

Description:
The Free Estriol (also referred to as unconjugated estriol or uE3 in the literature) ELISA is a competitive immunoassay. Competition occurs between Estriol present in calibrators, controls and patient samples and an enzyme-labelled antigen (conjugate) for a limiting number of anti-Estriol antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added, and approximately 15–20 minutes later the enzymatic reaction is terminated by addition of stopping solution. The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of Estriol in the sample. A calibrator curve is plotted with a provided set of calibrators to calculate directly the concentration of estriol in patient samples and controls. Serum and salivary assays follow the same procedure except that the volume of the calibrators, controls and samples dispensed into the microplate wells is 10 µL for serum assays and 20 µL for salivary assays.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Limit of detection: serum: 0.058 ng/ml; saliva: 0.034 ng/ml;
IVD