Cookies help us deliver our services. By using our services, you agree to our use of cookies.
Products specifications
| Technology | Other |
| Sizes | 1mL (20.000U) |
Description
Ribonuclease A is a pancreatic endoribunuclease that specifically cleaves single-stranded RNA at the 3’ end of pyrimidine residues. Lyophilized powder purified from bovine pancreas.
PRODUCT: Exonuclease I (Exo I) is an exonuclease that hydrolyzes single-stranded DNA, one nucleotide at a time from the end, in the 3´→5´ direction. It releases 5´- mononucleotides one after another and leaves the terminal 5´-dinucleotide intact. This 55kDa enzyme, product of the E.coli sbcB gene, does not cleave DNA strands without terminal 3´-hydroxyl groups, as these are blocked by phosphoryl or acetyl groups1. It does not degrade double-stranded DNA. For activity, Exo I requires magnesium (optimal [Mg2+] is 10mM). Exo I is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to most molecular biology buffers containing magnesium (>1mM), including PCR reaction mixtures.
PRODUCT: Exonuclease I (Exo I) is an exonuclease that hydrolyzes single-stranded DNA, one nucleotide at a time from the end, in the 3´→5´ direction. It releases 5´- mononucleotides one after another and leaves the terminal 5´-dinucleotide intact. This 55kDa enzyme, product of the E.coli sbcB gene, does not cleave DNA strands without terminal 3´-hydroxyl groups, as these are blocked by phosphoryl or acetyl groups1. It does not degrade double-stranded DNA. For activity, Exo I requires magnesium (optimal [Mg2+] is 10mM). Exo I is tolerant to a wide variety of buffer conditions (salt, pH, etc), and thus can be added directly to most molecular biology buffers containing magnesium (>1mM), including PCR reaction mixtures.
Similar Products
Dnase I Set (for 100 prep kits)
Incubation with the RNase-free DNase I set ensures complete DNA removal from RNA solutions as this enzyme hydrolyzes phosphodiester bonds of dsDNA, ssDNA, chromatin, and DNA-RNA hybrid molecules, without compromising RNA yield and integrity.
0.9219328835606575
RNAse A (Proteinase free lyophilized >50KU/mg)
Ribonuclease A is a pancreatic endoribunuclease that specifically cleaves single-stranded RNA at the 3’ end of pyrimidine residues. Lyophilized powder purified from bovine pancreas
0.906459391117096
RNase A (50mg/ml)
RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues.
0.8863206133246422
RNase A (10mg/ml)
RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues.
0.8863206133246422
BCA Quantification Kit (High Sensitivity)
GRiSP´s BCA Quantification Kit (High Specificity) is formulated for the quantification of total protein concentration ranging from 0.5-20µg/ml. It is suitable for usage with standard spectrophotometers as well as with microplate readers.