ELISA Test Foot and Mouth Disease virus (FMDV) (multi-species)

ELISA kit for the detection of antibodies against Foot-and-mouth disease virus (FMDV) in bovine, goat, ovine, and porcine serum

DESCRIPTION:
This kit is based on an immunoenzymatic assay for the detection of antibodies against Foot-and-mouth disease virus (FMDV) in bovine, ovine and porcine serum.

SUMMARY AND EXPLANATION:
FMD is a highly contagious infection which affects cloven-hoofed domesticated and wild ruminants such as cattle, sheep, goat, as well as pigs. The agent responsible for this disease is the FMDV of which seven serotypes have been identified to date: O, A, C, Asia 1, SAT 1, 2, and 3. FMD is a significant constraint to international trade in live animals and animal products. The FMDV genome encodes a single polyprotein from which the different viral polypeptides are cleaved by viral proteases, including eight different non-structural proteins (NSPs). Both structural and non-structural antigens induce the production of antibodies in infected animals.
Control of the disease in FMD-free countries includes movement restrictions and a slaughter policy. However, elimination of infected and contact animals alone may not be sufficient to eradicate the virus and emergency vaccination may be considered as an additional option. Identifying animals either vaccinated or unvaccinated that have had contact with live virus, from those that have been only vaccinated against the disease is of considerable importance since both groups have neutralizing antibodies in their sera. Using traditional serological techniques, it is not possible to distinguish FMD infected animals from vaccinated animals and control authorities have limited possibilities to monitor virus presence or circulation. By contrast blocking ELISA based on recombinant FMDV 3ABC antigen (non-structural protein) and a 3B monoclonal antibody allows differentiating infected from vaccinated animals (DIVA), depending of the purity of the vaccine.

CHARACTERISTICS:
- Blocking ELISA: uses recombinant 3ABC protein of FMDV and an anti-3B monoclonal antibody (mAb) conjugated to horseradish peroxidase (HRPO)
- structural 3B antigen
- 5 breakable plates/kit
- Tests per kit: up to 450 samples