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Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | BIOPEXT-0609.250: 250 preps BIOPEXT-0609.50: 50 preps |
| Technical Sheet | Download PDF |
Description
DNA extraction and purification from food samples (plant or animal origin and bacteria), stool and bacterial DNA using spin columns
PRODUCT DESCRIPTION: The Biopremier DNA Extraction Kit from Food provides an efficient procedure for the isolation and purification of total DNA from food samples (raw material and processed food of animal or plant origin), stool and bacteria. After the food, feed and stool samples have been homogenized, the DNA can be extracted with the lysis buffer. In case to detect bacterial DNA samples, a pre-enrichment with the appropriate culture medium is recommended. The bacterial DNA can be extracted with the lysis buffer, after a concentration step by centrifugation.
Lysis occurs by thermal shock and the purification by a series of processes to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the binding buffer, to create conditions for optimal binding to the silica membrane column. The purified DNA is ready-to-use for subsequent reactions like real-time PCR detection and quantification.
PRODUCT FEATURES:
-DNA from stool samples
-DNA from complex matrices, processed food, raw food and animal feed, soya, chocolate, cereals, meat
-Extraction of microbial DNA
-DNA suitable for PCR, real-time PCR, enzymatic reactions
-Applications: to detect specific DNA in animal feed
-Applications: to detect genetically modified material in food products (OGM)
-Compatible with BIOPREMIER Real time detetion kits and others Real Time detection / End-Point Kits
-Available formats: Kits with 250 or 50 preps
PRODUCT DESCRIPTION: The Biopremier DNA Extraction Kit from Food provides an efficient procedure for the isolation and purification of total DNA from food samples (raw material and processed food of animal or plant origin), stool and bacteria. After the food, feed and stool samples have been homogenized, the DNA can be extracted with the lysis buffer. In case to detect bacterial DNA samples, a pre-enrichment with the appropriate culture medium is recommended. The bacterial DNA can be extracted with the lysis buffer, after a concentration step by centrifugation.
Lysis occurs by thermal shock and the purification by a series of processes to remove contaminants and residual cellular debris. The clear supernatant is then mixed with the binding buffer, to create conditions for optimal binding to the silica membrane column. The purified DNA is ready-to-use for subsequent reactions like real-time PCR detection and quantification.
PRODUCT FEATURES:
-DNA from stool samples
-DNA from complex matrices, processed food, raw food and animal feed, soya, chocolate, cereals, meat
-Extraction of microbial DNA
-DNA suitable for PCR, real-time PCR, enzymatic reactions
-Applications: to detect specific DNA in animal feed
-Applications: to detect genetically modified material in food products (OGM)
-Compatible with BIOPREMIER Real time detetion kits and others Real Time detection / End-Point Kits
-Available formats: Kits with 250 or 50 preps
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