BactoReal® Kit Mycobacterium avium ssp. paratuberculosis

Detection of the IS900 region of Mycobacterium avium subspecies paratuberculosis

Background: Mycobacterium avium ssp. paratuberculosis (MAP) causes Johne's disease, a chronic gastroenteritis affecting primarily ruminants and many other species. This disease is one of the most widespread and economically important diseases of ruminants. It has a relatively long incubation period characterized by the excretion of MAP in faeces for months and years before first clinical symptoms develop.

Description: BactoReal® Kit Mycobacterium avium ssp. paratuberculosis uses real-time PCR for the detection of the insertion sequence IS900 of M. avium ssp. paratuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: IS900 of Mycobacterium avium ssp. paratuberculosis
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX