BactoReal® Kit Leptospira spp. Multiplex (16S rDNA + LipL32)

Brand: Ingenetix
Detection and differentiation of LipL32 gene of pathogenic and 16S rRNA gene of pathogenic and intermediately pathogenic Leptospira species
SKU: DVEB00713

Register or Log In in order to request a quote

Register Login

Products specifications
TechnologyqPCR / Real Time PCR/ PCR
SizesDVEB00713: 100 reactions - FAM, VIC/HEX, Cy5
DVEB00753: 50 reactions - FAM, VIC/HEX, Cy5
Detection and differentiation of LipL32 gene of pathogenic and 16S rRNA gene of pathogenic and intermediately pathogenic Leptospira species

Background: The spirochaetal Leptospira genus consists of pathogenic species (L. interrogans, L. noguchii, L. weilii, L. kirschneri, L. alexanderi, L. borgpetersenii, L. santarosai, L. kmetyi, Leptospira genomospecies 1), of intermediately pathogenic species (L. inadai, L. fainei, L. broomii, L. licerasiae, L. wolffii) and of non- pathogenic species (L. biflexa, L. meyeri, L. wolbachii, Leptospira genomospecies 3, 4 and 5). Members of Leptospira can also be grouped into serovars. Currently over 200 serovars are recognized in the genus Leptospira and a few serovars are found in more than one species of Leptospira. Non-pathogenic species are saprophytes and can grow outside the host animal, while pathogenic Leptospira species cause leptospirosis. They affect many mammalian species, including humans. Animals may become unapparent carriers, and shedding of leptospires, primarily in the urine, serves as a source of infection for other animals and humans. The clinical signs associated with leptospirosis are variable and depend on the infecting serovar and the susceptibility of the animal. Leptospires are present in the blood during the first 5 to 10 days after onset of the disease. Only pathogenic Leptospira species express an outer membrane lipoprotein encoded by the LipL32 gene.

Description: BactoReal® Kit Leptospira spp. Multiplex (16S+LipL32) uses multiplex real-time PCR for the detection and differentiation of the LipL32 gene of pathogenic Leptospira species as well as of the 16S rDNA gene of both intermediately pathogenic and pathogenic Leptospira species. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 16S rDNA gene and LipL32 gene of Leptospira spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5
Similar Products

BactoReal® Kit Leptospira spp. (16S rDNA)

Detection of the 16S rRNA gene of pathogenic and intermediately pathogenic Leptospira species

REAL TIME PCR DETECTION TEST KIT Bovichek® Mycoplasma spp and M. bovis qPCR

This qPCR kit allows detection of Mycoplasma spp. and M. bovis in bovine sample

Real Time PCR Detection Kit Leptospira spp.

Leptospira species detection test kit by using qPCR. Leptospira is a genus of bacteria that causes Leptospirosis (also known as Weil’s syndrome, canicola fever, canefield fever, nanukayami fever, 7-day fever, Rat Catcher’s Yellows, Fort Bragg fever, black jaundice, and Pretibial fever) and affects humans as well as other animals. The infection is commonly transmitted to humans by allowing water that has been contaminated by animal urine to come in contact with unhealed breaks in the skin, the eyes, or the mucous membranes.

Real Time PCR Detection Kit Mycoplasma spp.

Mycoplasma spp. detection test kit by using qPCR. Mycoplasma parasitize, on the surface or intracellularly, a wide range of organisms including humans, plants, animals, and insects. When Mycoplasma acts as a pathogen they cause chronic but mild infections that rarely kill their host.

Real Time PCR Detection Kit Mycoplasmas Mycoides Cluster Panel (M. mycoides M. capricolum and M. putrefaciens)

Mycoplasma mycoides cluster Mycoplasma mycoides, Mycoplasma capricolum and Mycoplasma putrefaciens detection test kit by using qPCR. Mycoplasma mycoides cluster is a group of closely related infectious mycoplasmas. Comprises the ruminant pathogens M. mycoides subsp. mycoides the causative agent of contagious bovine pleuropneumonia, M. capricolum subsp. capripneumoniae the agent of contagious caprine pleuropneumonia, M. capricolum subsp. capricolum, M. leachii and M. mycoides subsp. capri. M. putrefaciens is related to contagious agalactia together with some subspecies of M. capricolum and M. mycoides. Contagious bovine and caprine pleuropneumonia are major livestock diseases and impact the agricultural sector, especially in developing countries.