BactoReal® Kit European Foulbrood -Melissococcus plutonius

Detection of the 16S rRNA gene of Melissococcus plutonius

Background: European foulbrood (EFB) is a bacterial brood disease of honeybees caused by the Grampositive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae are infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Larvae that die from the disease have been starved of food.

Description: BactoReal® Kit European Foulbrood uses real-time PCR for the detection of the 16S rRNA gene of Melissococcus plutonius. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 16S rRNA gene of Melissococcus plutonius
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX