Cookies help us deliver our services. By using our services, you agree to our use of cookies.
Products specifications
| Technology | qPCR / Real Time PCR/ PCR |
| Sizes | DVEB04013: 100 reactions - FAM, VIC/HEX, Cy5 DVEB04053: 50 reactions - FAM, VIC/HEX, Cy5 |
Description
Detection of virulence-associated genes stx1 and stx2 (verotoxin) of Escherichia coli
Background: Escherichia coli is a Gramnegative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors, which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. Enteropathogenic Escherichia coli (EPEC) produces an outer membrane protein called intimin, encoded by eae and mediating intestinal cell attachment. Intimin is a virulence factor (adhesin) of EPEC and EHEC strains. Furthermore, EPEC are classified into typical and atypical strains based on the presence of the plasmid E. coli adherence factor (EAF). Typical EPEC strains are rarely isolated from animals, and humans are the major natural reservoir. The reservoir for atypical EPEC can be both animals and humans. By definition all EPEC lack genes to produce shiga toxin (stx).
Description: BactoReal® Kit E. coli Typing is a series of different real-time PCR kits for detection of virulence-associated genes of E. coli. Depending on which kit and which combinations of kits are used, they allow the typing of enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and of Shiga toxin (verotoxin) producing E. coli (STEC). Assays for stx1 and stx2, as well as assays for eae and ehxA can be combined in a multiplex PCR. All kits consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: virulence-associated genes stx1 & stx2 of E. coli
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5
Background: Escherichia coli is a Gramnegative bacterium that is a member of the normal gut flora of humans and animals. Most E. coli strains are harmless, but some serotypes carry virulence factors, which enable them to be pathogenic in target hosts. These pathogenic E. coli are implicated in a number of diseases including diarrhoea, septicaemia and urinary tract infections in humans and animals. Enteropathogenic Escherichia coli (EPEC) produces an outer membrane protein called intimin, encoded by eae and mediating intestinal cell attachment. Intimin is a virulence factor (adhesin) of EPEC and EHEC strains. Furthermore, EPEC are classified into typical and atypical strains based on the presence of the plasmid E. coli adherence factor (EAF). Typical EPEC strains are rarely isolated from animals, and humans are the major natural reservoir. The reservoir for atypical EPEC can be both animals and humans. By definition all EPEC lack genes to produce shiga toxin (stx).
Description: BactoReal® Kit E. coli Typing is a series of different real-time PCR kits for detection of virulence-associated genes of E. coli. Depending on which kit and which combinations of kits are used, they allow the typing of enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC) and of Shiga toxin (verotoxin) producing E. coli (STEC). Assays for stx1 and stx2, as well as assays for eae and ehxA can be combined in a multiplex PCR. All kits consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Product Features:
- Amplification and detection: virulence-associated genes stx1 & stx2 of E. coli
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5
Similar Products
BactoReal® Kit E. coli Typing (EHEC EPEC) eae & ehxA
Detection of virulence-associated genes eae (intimin) and ehxA (enterohemolysin) of Escherichia coli
0.9674169085919857
BactoReal® Kit E. coli Typing (EHEC or EPEC) eae
Detection of virulence-associated gene eae (intimin) of Escherichia coli
0.9624477289617062
BactoReal® Kit E. coli Typing (EHEC) ehxA
Detection of virulence-associated gene ehxA (enterohemolysin) of Escherichia coli
0.9557033702731133
Real Time PCR Detection Test Kit Escherichia Coli Supreme (Stx1 Stx2 and Eae Genes)
Food PCR test detection of E. coli pathogenic genes (stx1 , stx2 and eae) in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136:2012 (recommended). For use in real time instruments with FAM and ROX channels.
0.9544330537319183
Condagene STEC ID
Real Time Pcr Detection Test Kit Escherichia Coli Serotype / Serogroup (O157 O26 O111 O103 And O145). Food PCR test detection of E. coli serotypes O157, O26, O111, O103, and O145 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, and ROX channels.