5-HIAA ELISA

Background:5-Hydroxyindolacetic acid (5-HIAA) is a metabolite of the serotonin pathway [1, 2]. Serotonin and its major urinary metabolite 5-HIAA, is produced in excess by most enterochromaffin cells from carcinoid tumors, especially those associated with the carcinoid syndrome. Several studies and publications show that analysis of urinary 5-HIAA is important in diagnosis of carcinoid patients [1-8]. Therapeutic consequences should never be based on laboratory results alone, even if these results are assessed in accordance with the quality criteria of the method. Any laboratory result is only a part of the total clinical picture of the patient. Only in cases where the laboratory results are in an acceptable agreement with the overall clinical picture of the patient, it can be used for therapeutic consequences.

Description:
Enzyme immunoassay for the quantitative determination of 5-Hydroxyindolacetic acid (5-HIAA) in urine. The determination of 5-HIAA helps in the diagnosis of carcinoids. The quantitative determination of 5-HIAA follows the basic principles of a competitive enzyme immunoassay. First, 5-HIAA is chemically derivatized by a methylation step. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The methylated analyte in the standards, controls and samples compete with the solid phase bound analyte for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigenantibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate resulting in a colour reaction. The reaction is monitored at a wavelength of 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. Manual processing of the ELISA is recommended. The use of automatic laboratory equipment is the responsibility of the user. This in-vitro diagnostic is for professional use only.

Product features:
The kit contains reagents for 96 determinations;

Microtiter plate reader at 450 nm and if possible 620-650 nm;
Assay range: 0.4 - 50 mg/L;
Limit of detection: 0,23 mg/l;
IVD