ViroTyping Kit InfA H5N8

qPCR / Real Time PCR/ PCR
50 reactions

Background: Influenza A virus is a single-stranded, negative-sense RNA virus of the family Orthomyxoviridae. Of the three Influenza genera (A, B, C), only Influenza A is known to infect birds. Avian Influenza is further characterized based on the presence of haemagglutinin/neuroaminidase subtypes. Various strains, especially of H5N1 and H5N8 genotype, are responsible for outbreaks in domestic ducks and migratory birds in East Asia, Northern America, parts of Europe and the Middle East. Further spreading around the globe is possible through migratory birds. Virulence potential of a certain strain can vary from mild to severe due to high sequence variability.

Description: ViroTyping Kit InfA H5N8 contains two PCR primer sets for the amplification and detection of RNA of avian influenza A H5N8. This test is based on an intercalating dye using one-step reverse-transcription real-time PCR and was developed and validated for the Applied Biosystems® 7500 (Fast) instrument (Thermo Fisher Scientific), but is also suitable for other real-time PCR instruments. This test allows the rapid typing of avian influenza H5N8 from RNA samples. ViroTyping Kit InfA H5N8 detects the haemagglutinin (H) and neuraminidase (N) genes of avian influenza A virus, subtype H5N8. Amplification curves and specific melting curves for the targets H5 and N8 indicate the amplification of genotype specific RNA. It should be emphasized, however, that the viral genome consists of segmented RNA fragments highly prone to sequence variation. The test is based on currently published viral motives of known virulence, but constant re-arrangement makes it impossible to cover all potential relevant strains. Master mix and separate positive controls for Influenza A H5 and Influenza A N8 are included.

Product Features: -ViroTyping Kit InfA H5N8- for Genotyping of avian Influenza A positive samples (pre-screening recommended) -Real-time PCR and Melting Curve Analysis - master mix included -Specific positive controls for H5 and N8
- ROX™ dye as passive reference