ViroReal® Kit VZV (varicella zoster virus)

qPCR / Real Time PCR/ PCR
50 reactions
ViroReal® Kit VZV (varicella zoster virus)

Detection of the ORF29 of varicella zoster virus.

Background: Varicella zoster virus (VZV), also called human herpes virus type 3 (HHV-3) is a double-stranded DNA virus. The VZV is found worldwide (> 95% of persons are seropositive) and is transmitted by droplet infection. Primary infection with VZV causes chickenpox. After primary infection, VZV remains dormant in sensory nerve roots for life. Upon reactivation, the virus migrates down the sensory nerve to the skin, causing herpes zoster (also called shingles). Shingles generally affects the elderly and people with immunodeficiency.

Description: ViroReal® Kit VZV uses real-time PCR for the detection of the ORF29 gene coding for a single-stranded DNA-binding protein gene of VZV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: ORF29 of VZV
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or assay-only