ViroReal® Kit Parvovirus B19

qPCR / Real Time PCR/ PCR
50 reactions
ViroReal® Kit Parvovirus B19

Detection of the VP1 gene of parvovirus B19 genotypes 1, 2 and 3.

Background: Parvovirus B19 is a linear single stranded DNA virus. The genetic diversity of the found B19 strains falls into the three well-recognized genotypes 1, 2 and 3 with approximately 10-15% nucleotide divergence between them. The virus is primarily spread by infected respiratory droplets, but also blood-borne or transplancentar transmission is possible. Infection with parvovirus B19 can cause erythema infectiosum, which is a childhood exanthem more commonly known as fifth disease, furthermore it might cause aplastic crisis, hydrops fetalis and arthropathy, but 25% of cases can remain asymptomatic.

Description: ViroReal® Kit Parvovirus B19 uses RT real-time PCR for the detection of the VP1 gene of the parvovirus B19 genotypes 1, 2 and 3. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: VP1 gene of Parvovirus B19
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or assay-only