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Real Time Pcr Detection Test Kit Escherichia Coli Serotype / Serogroup (O157, O26, O111, O103, And O145)

BIOPSFS-0045
qPCR / Real Time PCR/ PCR
Biopremier
250 (25 reactions each)
(pdf) Instructions for use
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Real Time Pcr Detection Test Kit Escherichia Coli Serotype / Serogroup (O157, O26, O111, O103, And O145)

Detection of E. coli serotypes O157, O26, O111, O103, and O145 in food products, animal feedstuff and environmental samples. Pre-enrichment according to ISO/TS 13136 is recommended or from E. coli isolated colonies. For use in real time instruments with FAM, and ROX channels.

BACKGROUND: Escherichia coli (E. coli) is a bacterium that is commonly found in the gut of humans and warm-blooded animals and most of the strains are harmless. Some strains however, such as enterohemorrhagic E. coli (EHEC) can cause serious foodborne outbreaks that causes diarrhoea, fever and vomiting in humans.

The need for rapid, accurate, and sensitive methods for the detection of these E. coli strains is a major food safety issue. Since conventional microbiological methods for their detection and identification are time-consuming. Today, there are approved methods for PCR based detection of pathogenic genes of Escherichia coli unique DNA sequences, in particular using real time PCR and specific fluorescent probes.

PRODUCT DESCRIPTION: SUPREME REAL TIME DETECTION E.coli serotype / serogroup (O157, O26, O111, O103, and O145) uses real-time PCR based on the use of TaqMan technology for the detection of pathogenic E. coli, providing a simple, reliable and rapid result for the detection of the diverse E. coli serotypes.
The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target. Our carefully designed primers and probe ensure highest sensitivity and specificity.

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