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REAL TIME PCR DETECTION TEST KIT beverages Brettanomyces/Dekkera
Item code
BIOPFS-0019
Technology
qPCR / Real Time PCR/ PCR
Manufacturer
Biopremier
Variants
BIOPFS-0019: 100 reactions - FAM, ROX
BIOPFS-sp0019: 10 reactions - FAM, ROX
Instructions
(pdf)
Instructions for use
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Detection Brettanomyces bruxellensis in wine and other beverages. The kit is suitable for use in real time instruments with FAM and ROX detection channels.
BACKGROUND:
The yeast Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis) is a spoilage yeast found in wine and various alcoholic beverages and can be found on grape berries, cellar equipment, and barrels. B. bruxellensis has been described as the principal-agent responsible for the formation of volatile phenols namely 4-ethylphenol and 4-ethylguaiacol which leads to the characteristic “Brett” taints associated with red wine, developed during wine aging and bottling. At this stage, the wines are poorly protected via the appropriate use of fungicides and due to the resistance of B. bruxellensis to sulfur dioxide control of these yeasts becomes difficult. Moreover, B. bruxellensis viability in wine seems to be enhanced by its ability to use alternative carbon sources, therefore being able to survive in the wine long after fermentation. Detection of B. bruxellensis in wine is usually performed using traditional microbiological techniques, based on culturing the organism on appropriate media and incubation for 1–2 weeks, which can have important economic consequences to wine and alcoholic beverages industry. Thus, in vitro amplification of DNA by polymerase chain reaction (PCR), in particular, using real time PCR and specific fluorescent probes, is a powerful and reliable alternative for B. bruxellensis detection, providing results in much shorter times and allowing more efficient control procedures.
PRODUCT DESCRIPTION:
REAL TIME DETECTION KIT Brettanomyces/Dekkera bruxellensis uses real-time PCR for the detection of Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis) in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the target microrganism as well as a positive control and an internal control (IC).
PRODUCT FEATURES:
-Amplification and detection of Brettanomyces bruxellensis (teleomorph Dekkera bruxellensis)
-Real-time PCR with rapid hot-start Taq DNA polymerase
-Internal Control to exclude false-negative results
-PCR- platforms: runs on all established standard real-time PCR- platforms
-Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously
-Channel Target: FAM, Channel IC: ROX
-Available formats: Kits with 100 or 10 reactions
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Equipments
Agitators - Incubators - Vortex
Autoclave Dry Heat Sterilizer
Automatic Pipettors - Liquid dispensers
Balances
Baths - Heating thermoblock
Centrifuges
Electrodes pH - Conductivity - Oxygen
Electrophoresis
Equipments ELISA
Equipments Lateral flow
Equipments Real Time PCR
Freezers - Refrigerators
Homogenizer - Food homogenizer
Microbiological incubators - Chambers
Micropipettes - Electronic Pipettes
Microscopes
PCR - Laminar Flow Chambers
Spectrophotometers
Ultrasounds - Sonicators
Water Purifier
Food
Beverages quality
Food adulteration
Food allergens
Food safety
Genetically modified organisms (GMO)
Mycotoxins
Plant pathogens
Water quality
Other food tests
Human
Bacteria
Covid-19 tests
Fungi
Parasites
Virus
General laboratory solutions
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Cloning and cell research
Culture media
DNA/RNA amplification and analysis
DNA/RNA extraction and purification
Plasticware
Veterinary
Bacteria
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Virus
Other microrganisms
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