qPCR / Real Time PCR/ PCR
50/50 or 5/5 reactions

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Please choose the number of reactions

BACKGROUND: Vibrio cholerae is a waterborne and food-born pathogen that causes gastrointestinal disorders with a wide range of clinical manifestations, including vomiting and rice-like diarrhea. On the other hand, Vibrio parahaemolyticus is found worldwide in estuarine waters and is a leading cause of gastroenteritis. Typically, human infections with these organisms result from the consumption of raw or undercooked seafood. In the case of V. cholerae infections by contaminated water, ingestion is also relevant. The detection and identification of these species are usually made based on culture and biochemical tests that are time-consuming and laborious and sometimes not accurate. Therefore, an effective method for the detection of both V. cholerae and V. parahaemolyticus is mandatory. In vitro amplification of DNA by the polymerase chain reaction (PCR) has become a powerful alternative in microbiological diagnostics due to its rapidity and accuracy, in particular using real time PCR and specific fluorescent probes. PRODUCT DESCRIPTION: REAL TIME DETECTION KIT Vibrio spp. uses real-time PCR for the detection of Vibrio cholerae and Vibrio parahaemolyticus in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target microorganism. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal control (IC). PRODUCT FEATURES: -Amplification and detection of Vibrio cholerae and Vibrio parahaemolyticus -Real-time PCR with rapid hot-start Taq DNA polymerase -Internal Control to exclude false-negative results -PCR- platforms: runs on all established standard real-time PCR- platforms -Harmonized thermal profiles to run with other BIOPREMIER kits simultaneously -Channel Pathogen: FAM, Channel IC: ROX -Available formats: Kits with 50 reactions for each target or 5 reactions for each target

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