NEW : SUPREME REAL TIME DETECTION KIT E. coli (Eae and Stx pathogenic genes)- 100 rxn


Advantages of the new SUPREME product line are:

- Lower contamination risk

- Higher sensitivity and specificity

- UNG enzyme incorporated

- Higher enzyme stability over time

- New packaging


Each kit contains 5 tubes: 2x Master Mix, 2x Enzymes and Positive Control


Enteropathogenic E. coli (EPEC) was the first recognized pathogenic group, and presently,continues to be a leading cause of diarrhoea among infants from developing countriesworldwide. The locus of enterocyte eacement (LEE) is necessary for the pathogenicity andintimin one of the resultant products, coded by eae gene1. The main pathogenic property of Shiga toxin-producing E. coli (STEC) strains is the productionof Shiga toxins, coded by stx genes. Illnesses associated with STEC are usually watery diarrhoeaand are particularly serious in children. Numerous outbreaks have been attributed to STEC strains of serotype O157:H7, but non-O157serogroups, most commonly O26, O55, O103, O111, O117 O145, O146 and O191 have beenshown to cause infections in the EU over recent years. The focus on E. coli O157 instead of other serotypes has been further enhanced by the ease of isolation of E. coli O157. In contrast, the isolation of other serotypes has been sub-diagnosed by the lack of suitable methods to isolate those strains. EHEC were originally defined as a subset of STEC, that were associated with watery diarrhoea,haemorrhagic colitis, haemolytic-uremic syndrome, and that in addition to the stx-encodinggenes, usually carry the attaching and effacing gene (eae; intimin-coding). EHEC strains aretypically isolated from cases of severe disease but are poorly defined because there is no commonly accepted definition of EHEC.

SUPREME E.coli (Eae and Stx pathogenic genes)

  • SUPREME REAL TIME DETECTION KIT E. coli is a kit for the detection of pathogenic E. coli associated with the pathotypes EPEC, STEC and the sub-group EHEC associated with the combination of the virulence genes stx1 and/or stx2 and eae. Within Enterobacteriaceae other species may also contain these virulence genes, therefore, the kit also detect species like E. albertii, Shigella boydii or S. sonnei, when target genes are present. The kit enables a qualitative detection of target genes in food samples by Real Time PCR, after a selective enrichment step. The test may be used with the following matrixes: raw ground beef, orange juice, salad (green, purple lettuce and coriander) and cream cheese.

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