REAL TIME DETECTION DUPLEX KIT Legionella spp. and L. pneumophila SAMPLE-10 rxn
|Each kit contains: Master Mix, Assay Mix, Positive and Negative Controls|
|Nowadays, 47 species and more than 60 serogroups of Legionella spp. have been recognized. Legionnaires’ disease is the most severe form of infection, which includes pneumonia, and the fatality rate can approach 50% in immunocompromised patients. Outbreaks of Legionnaires’ disease have been worldwide traced to a wide variety of environmental water sources such as cooling towers, hot tubs, showerheads, whirlpools and spas and public fountains. Over the last few years, the reported incidence of legionellosis has steadily increased. Commonly used method for environmental surveillance of Legionella spp. is the standard culture technique, but it has several limitations as it requires selective media and prolonged incubation periods. Recently, rapid and sensitive alternative methods have been found to be attractive alternatives to the conventional culture method for the detection of slow-growing and fastidious bacteria such as Legionella spp. These methods are PCR based methods, in particular real time PCR based, that allows the rapid detection Legionella spp. in water samples.|
DUPLEX KIT Legionella spp. and L. pneumophila SAMPLE
This test allows the detection of Legionella spp. and L. pneumophila in water samples after concentration by membrane filtration and DNA extraction. It can also be used with other environmental samples. Water samples shall be concentrated according to ISO 11731:1998. The kit is suitable for use in real time PCR instruments with FAM and ROX detection channels.