REAL TIME DETECTION KIT L. pneumophila - 100 rxn
|Each kit contains: Master Mix, Assay Mix, Positive and Negative Controls|
|Legionella pneumophila is a causative agent of Legionnaires’ disease. It was first recognized in 1977 following an epidemic of acute pneumonia among veterans of the American Legion in Philadelphia and led to the discovery of a new bacterial species and genus. Nowadays, 47 species and more than 60 serogroups have been recognized. The vast majority of Legionnaires’ disease cases are due to L. pneumophila, being serogroup 1 responsible for up to 80% of reported cases. Legionnaires’ disease is the most severe form of infection, which includes pneumonia, and the fatality rate can approach 50% in immunocompromised patients. Outbreaks of Legionnaires’ disease have been worldwide traced to a wide variety of environmental water sources such as cooling towers, hot tubs, showerheads, whirlpools and spas, and public fountains. Over the last few years, the reported incidence of legionellosis has steadily increased. Commonly used method for environmental surveillance of Legionella is the standard culture technique, but it has several limitations as it requires selective media and prolonged incubation periods. Recently, rapid and sensitive alternative methods have been found to be attractive alternatives to the conventional culture method for the detection of slow-growing and fastidious bacteria such as Legionella. These methods are PCR based methods, in particular real time PCR based, that allows the rapid detection L. pneumophila in water samples.|
This test allows the detection of L. pneumophila in water samples after concentration by membrane filtration and DNA extraction. It can also be used with other environmental samples. Water samples shall be concentrated according to ISO 11731:1998. The kit is suitable for use in real time instruments with FAM and ROX detection channels.