REAL TIME DETECTION KIT L. Monocytogenes SAMPLE - 10 rxn
|Each kit contains: Master Mix, Assay Mix, Positive and Negative Controls|
|Listeria monocytogenes, the causative agent of listeriosis, is of clinical relevance for humans Listeriosis mainly a_ects specific population groups, with increased susceptibility: young, old, pregnant and immunodepressed. Normally Listeria is transferred to humans through raw milk, soft-ripened cheeses, raw vegetables, poultry, raw meats and raw or smoked fish. The ability of Listeria spp. to grow at low temperatures (3ºC) increases the risk of infection. The need for rapid, accurate and sensitive methods for the detection of Listeria is a major food safety issue. Since conventional microbiological methods for their detection and identification are time-consuming, PCR base detection of Listeria monocytogenes unique DNA sequences, due to its rapidity and accuracy, in particular using real time PCR and specific fluorescent probes.|
L. monocytogenes SAMPLE
This test allows the detection of L. monocytogenes in food products after enrichment in buffered peptone water and DNA extraction. It can also be used with animal feedstuff and environmental samples. Recommended a pre-enrichment according to ISO 11290. The kit is suitable for use in real time instruments with FAM and ROX detection channels.