REAL TIME DETECTION KIT Horse - 100 rxn
|Each kit contains: Master Mix, Assay Mix, Positive and Negative Controls|
|According to the European Commission Directive 2002/86/EC, food ingredients have to be declared. In case of meat and meat products, theanimal species has to be disclosed on the label. Moreover, the speciesauthenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non-meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue. Several methods for species detection are based are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR based techniques still allow the identification of DNA from the dfferent species present in a sample. Real time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity..|
This test allows the detection of Horse DNA in food products after DNA extraction. It can also be used with animal feedstuff and other samples in which horse species detection is needed. Depending on the specific characteristics of the sample, procedures like homogenization and grinding may be necessary before DNA extraction. The kit is suitable for use in real time instruments with FAM and ROX detection channels.