According to the European Commission Directive 2002/86/EC, food ingredients have to be declared. The fish species must be disclosed on the label with both commercial and scientific denominations in order to verify the origin and traceability of the fish and to analyze the quality control of handling and cleaning processes of production lines.
Methods for species detection based on the identification of proteins are not reliable for application in highly processed and heated products due to protein deterioration. DNA is more stable than proteins during processing . Real time PCR techniques are suitable for amplify small fragments of DNA high sensitivity and specificity.
At BIOPREMIER we developed The DNA Standard SUPREME that is designed to perform a comparative quantification analysis of fish DNA in food and feedstuff samples by Real Time PCR. By using the DNA Standard SUPREME, Real Time PCR users can include a precise low count of DNA in their assays. the DNA Standard SUPREME allows quantifications starting from 10% of the DNA, serving as a reference value to estimate fish authenticity.
DNA Standard SUPREME ATLANTIC COD (Gadus morhua)
Designed to perform a comparative quantitation analysis of Atlantic Cod (Gadus morhua) DNA in food and feedstuff samples by Real Time PCR. For reliable quantitative results it is recommended to apply the DNA Standard SUPREME Atlantic Cod (Gadus morhua) in combination with the SUPREME Real Time Detection Kit Atlantic Cod (Gadus morhua)