REAL TIME DETECTION KIT Brettanomyces/Dekkera bruxellensis - 100 rxn
|Each kit contains: Master Mix, Assay Mix, Positive and Negative Controls|
|The yeast Brettanomyces bruxellensis is a spoilage yeast found in wine and various alcoholic beverages and can be found on grape berries, cellar equipment and barrels. At this stage, the wines are poorly protected via the appropriate use of fungicides and due to the resistance of B. bruxellensis to sulphur dioxide control of these yeasts becomes difficult. Detection of B. bruxellensis in wine is usually performed using traditional microbiological techniques, based on culturing the organism on appropriate media and incubation for 1–2 weeks, which can have important economic consequences to wine and lcoholic beverages industry. Thus, real time PCR and specific fluorescent probes, is a powerful and reliable alternative for B. bruxellensis detection, providing results in much shorter times and allowing more efficient control procedures.|
This test allows the detection B. bruxellensis in wine and other beverages after DNA extraction. It can also be used with cultures and other samples in which B. bruxellensis detection is needed. Depending on the specific characteristics of the sample, procedures like homogenization and grinding may be necessary before DNA extraction. The kit is suitable for use in real time instruments with FAM and ROX detection channels.