Detection test kit for Canine disease pathogens detection by using qPCR: Ehrlichia spp., Anaplasma spp., Babesia spp., Leishmania spp., Brucella spp., Dirofilariimmiti, Hepatozoon spp., Mycoplasma haemofelis/haemocanis and Bartonella henselae. Infectious diseases in dogs are caused by a wide variety of infectious agents including viruses, bacteria, and parasites (protozoa and helminths). Most of them have their own morbidity and mortality in dogs and some of these infections are of zoonotic relevance. This panel is designed to detect most of the common infections in dogs as: Ehrlichia spp., Anaplasma spp., Babesia spp., Leishmania spp., Brucella spp., Dirofilaria immitis, Hepatozoon spp., Mycoplasma haemofelis/haemocanis and Bartonella henselae.