Detection test kit of Bovine ephemeral fever virus by using qPCR. Bovine ephemeral fever (BEF), commonly known as three-day sickness is a disease of cattle and occasionally buffaloes, marked by short fever, shivering, lameness, and muscular stiffness. It causes serious economic losses through deaths, loss of condition, decreased milk production, lowered fertility of bulls, mismothering of calves, delays in marketing, and restrictions on the export of live cattle.